MEDIFUGE & CGF KIT Patent pending - Blood Phase Separator
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Rotor Phase separator: 
The Silfradent Medifuge MF200 is a dedicated medical plasma centrifuge. It is designed to prepare autologous concentrated growth factor (CGF) and CD 34 + cells using patient's own blood wich is the ideal autologous source without the addition of exogenous substances. CGF represents a new generation of platelet that able to hold inside a higher concentration of autologus. 

Growth factors are proteins which regulate the complex processes of wound healing and enhance the body's healing abilities without side-effects. The Medifuge prepares up to 8 test tubes of various levels of concentrate with active proteins in liquified or Fibrin form to create membranes, glue or other particulars for tissue regeneration and bone augmentation in a short period of 12 minutes.

Medifuge MF200 Technical Specifications:

Product Features:
  • This unique medical device allows for the use of up to 8 test tubes for the creation of CGF (fibrin).
  • A micropocessor control system allows for the maintaining of a constant speed.
  • The exception rotor system with self-ventilation protects the blood sample from heat exposure. The rotor-holding compartment, the closing door and the test tube-holding jackets guarantee biological safety in terms of bio-containment, in the event of test tube breakage.
  • The test tube-holding jackets and rotor are built from thermal, antistatic material that is easy to clean, extract and sterilise in an autoclave at 135°.
  • MEDIFUGE is equipped with a decontamination cyclewith UVC reflected light.
  • Cycl duration 5 minutes at 1,000 revs.
  • The electronic control engine and its internal parts require no maintenance.
  •  Noise levels fall below the standards required and do not exceed 57 dBa.


MEDIFUGE & CGF KITPatent pending - Blood Phase Separator  
CGF KITThe patent kit is equipped with the neccessary for blood collection, specific dappens to prevent any contamination risks and the required instrument to produce the fibrin membrane and insertion of the clot in the site.

​Capacity: 8 units (test tubes)
Processing Time12 minutes
Power Source: 230V +/- 10%      50/60 Hz
Noise Level: Don't exceed 57 dB(A)
Dimensions of Unit: 230w x 320L x 240H mm
Weight: 9.4 Kg
Country of Manufacture: Italy







​Sifradent’s advance:
•Controlled acceleration
•Cycle Bleaching sterilizing= decontamination
•Antistatic, anti-magnetic rotor
•Self-ventilation to control temperature
•Deceleration control 
•Cooling 
•Temperature control 
•Self-decontamination 
•Rotor with alternate and controlled speed with an acceleration always under RCF300
Immagine

Blood Collection – Required Volume
Test tube PV 200R (unitary code) 10 ml
Glass plain test tube empty to produce CGF (Concentrated Growth Factors) solid Plasma.
Pack of 50 test tubes: single unit packed in double sterile bag.
Test tube PV 200P (unitary code) 10 ml
Glass test tube with separator gel and sodium citrate anticoagulant: to produce liquid plasma PRP (Platelet Rich Plasma).
Pack of 20 test tubes: single unit packed in double sterile bag.
Clicca qui per modificare.

VACUTAINER® Usage Based on Cap Color

 Red cap vacutainer: SOLID PHASE​
​WITH SILICON COATING

White cap vacutainer:  LIQUID PHASE (LPCGF)
​WITHOUT COATING OR ADDITIONAL SUBSTANCES

 Green cap vacutainer: LIQUID PHASE (LPCGF)
WITH SODIUM HEPARIN​
  • Used for creating membranes (can be reinforced with sticky membrane using white cap)
  • For preparing “el Poncho”
  • For clot used in socket preservation, filling bone cavities, sinus lift
    (must be cut like a star before condensing)
  • Mixed with autologous, heterologous, or artificial bone/bone substitute
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DENTAL CLINIC APPLICATION
  • CGF accelerates healing in sinus lift procedures and reduces recovery time with improved bone gain
  • Ridge augmentation using CGF
  • Socket preservation to maintain alveolar bone height
  • Intraosseous defect treatment and bone regeneration
  • Optimal for peri-implantitis
  • Cystic cavity filling
  • Treatment of furcation defects
  • Jaw reconstruction surgery
  • Combined periodontal-endodontic lesion treatment
  • Soft tissue grafts (gingival, subepithelial, etc.)
  • TMJ (Temporo-Mandibular Joint) regeneration
  • Other advanced dental regenerative procedures



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  • Intramuscular infiltrations
  • Sticky bone with autologous, heterologous, or artificial bone substitute
  • PPP layer for APAG (75°C)



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AESTHETIC AND COSMETIC APPLICATION
  • Full-face rejuvenation
  • Cellular regeneration
  • Facial volumization
  • Acne scar treatment
  • Aesthetic facial lipostructure
  • Removal of pigmented tissues/spots
  • Wrinkled neck and décolleté
  • Fine lines and wrinkles
  • Skin texture improvement
  • Treatment for dull, dry skin
  • Back of hands
  • Treatment for androgenetic alopecia or diffuse alopecia
  • Other aesthetic benefits
  • Intramuscular infiltrations (intravenous use also possible)
  • PPP layer for APAG
  • PRP layer for activated collagen (43°C)
  • Used in mesotherapy and electroporation
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ORTHOPEDIC APPLICATION
  • The purpose of using platelet-rich gel is to stimulate and accelerate the healing of connective tissues, reducing pain and enhancing both functional and reparative tissue formation.
  • Indicated for various articular, tendon, and muscular pathologies of the:
    • Knee
    • Shoulder
    • Wrist
    • Hip
    • Ankle
    • Elbow
  • Specific clinical indications include:
    • Osteoporosis
    • Tendinopathy
    • Epicondylitis
    • Epitrochleitis
    • Gonarthrosis (knee osteoarthritis)
    • Coxarthrosis (hip osteoarthritis)
    • Rhizoarthrosis (thumb base osteoarthritis)​
Each label shows the expiration date and the required quantity of blood to collect = 18 cc = 18 ml
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Centrifuge Balancing Instructions










Rotor Phase Separator – Silfradent’s Advance


  • Rotor made of antistatic and anti-magnetic material
    (No influence on Fe in red blood cells, minerals, heavy metals; reduces cancer-promoting substances – aflatoxins, cerium, polonium; eliminates Clostridium)
  • Differentiated acceleration
  • Speed and fractional – alternating RCF
    (prevents platelet degranulation)
  • Constant temperature maintained by self-ventilation
  • SELF-BLEACHING AND SELF-STERILIZING
  • Immediately after blood drawing (within 2 minutes), place the vacutainers opposite each other in the Medifuge.
  • Tubes with different cap colors can be centrifuged together.​

Warning: NEVER place uneven tubes!

The load must be well-balanced. Use sample tubes and balance tubes of identical weight or apply symmetrical placement patterns.
Any imbalance in mass can create dangerous force at high speeds, which may damage the rotor, the centrifuge, or cause injury.

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Blood Component Stratification Diagram  After Centrifugation – Common to Red, White, and Green Cap Tubes

This visual cross-section demonstrates the typical separation of blood components following centrifugation using the Medifuge system.
Although each cap color serves different clinical purposes, the stratification layers remain consistent across red, white, and green vacutainers.


Layer Structure & Volumes:
  • Serum (0.5 – 0.8 ml): Clear fluid at the top, platelet-poor.
  • Platelet Poor Fibrin-Rich Plasma (2 ml): Supports healing without promoting coagulation.
  • Platelet Rich Fibrin-Rich Plasma (2 ml): Rich in growth factors for tissue regeneration.
  • Buffy Coat/Clot Layer (0.7 ml): Contains a high concentration of CD34+ stem cells, crucial for cellular repair.
  • Red Blood Cells (RBC) (4 ml): Dense cellular component.
  • Cellular Fragments (5 ml): Residual content at the bottom.
The tube's content and coating (e.g., silicon or sodium heparin) determine whether a solid or liquid phase CGF is obtained and how long it can be preserved.
This diagram serves as a structural reference across the three tube types used in dental, aesthetic, and orthopedic regenerative protocols.

Note: The presence of a clearly defined CD34+ white layer highlights the high regenerative potential of this CGF protocol.